Journal: iScience

Article Title: Long noncoding RNA Lnc-DIF inhibits bone formation by sequestering miR-489-3p

doi: 10.1016/j.isci.2022.103949

Figure Lengend Snippet:

Article Snippet: Ser465/Ser467 phosphorylated SMAD2 Rabbit polyclonal antibody , Bioss , Cat# bs-3419R, RRID: AB_10880886.

Techniques: Luciferase, Plasmid Preparation, Recombinant, In Vivo, Transfection, Reporter Assay, In Situ Hybridization, Sequencing, Software

Journal: PLoS ONE

Article Title: Long-Term Gene Therapy with Thrombospondin 2 Inhibits TGF-β Activation, Inflammation and Angiogenesis in Chronic Allograft Nephropathy

doi: 10.1371/journal.pone.0083846

Figure Lengend Snippet: In gene therapy, treated rats with renal transplants TGF-β were evaluated by immunohistochemistry. Active TGF-β was either detected directly by using an antibody recognizing active TGF-β (A) or indirectly by evaluation of phosphorylation of the TGF-β signaling molecule smad2/3 (B) or expression of the TGF-β downstream target PAI-1 within the glomeruli (C) or the cortex (D). Representative microphotographs of PAI-1 staining in renal grafts treated with control (E) and TSP-2 overexpressing plasmid (F) are shown. Total TGF-β1 (G) and TGF-β2 (H) was similar in both groups. Control (n = 8) vs. TSP-2 treated (n = 8); *p<0,05.

Article Snippet: The TGF-β system was studied using antibodies to TGF-β1 (rabbit anti-human TGF-β1, Santa Cruz Biotechnology Inc. ); TGF-β2 (rabbit anti-human TGF-β2, Santa Cruz ); active TGF-β1 (chicken anti-human active TGF-β1, (R&D systems, Germany , , Phospho-Smad2/3 (rabbit anti-human Smad2 peptide phosphorylated at Ser-433/435, Santa Cruz ) or PAI-1, a rabbit polyclonal to human plasminogen activator inhibitor-1 (Santa Cruz).

Techniques: Immunohistochemistry, Phospho-proteomics, Expressing, Staining, Control, Plasmid Preparation

Journal: PLoS ONE

Article Title: Long-Term Gene Therapy with Thrombospondin 2 Inhibits TGF-β Activation, Inflammation and Angiogenesis in Chronic Allograft Nephropathy

doi: 10.1371/journal.pone.0083846

Figure Lengend Snippet: Representative microphotographs from immunohistological staining of kidney grafts for active TGF-β (A, control plasmid; B, TSP-2 therapy, brown cytosolic staining), P-smad 2/3 (C, control plasmid; D, TSP-2 therapy, brown nuclear staining), PAI-1 (E, control plasmid; F, TSP-2 therapy, brown staining), fibronectin (G, control plasmid; H, TSP-2 therapy, brown staining) and alpha-smooth muscle actin (I, control plasmid; J, TSP-2 therapy, brown staining) are shown.

Article Snippet: The TGF-β system was studied using antibodies to TGF-β1 (rabbit anti-human TGF-β1, Santa Cruz Biotechnology Inc. ); TGF-β2 (rabbit anti-human TGF-β2, Santa Cruz ); active TGF-β1 (chicken anti-human active TGF-β1, (R&D systems, Germany , , Phospho-Smad2/3 (rabbit anti-human Smad2 peptide phosphorylated at Ser-433/435, Santa Cruz ) or PAI-1, a rabbit polyclonal to human plasminogen activator inhibitor-1 (Santa Cruz).

Techniques: Staining, Control, Plasmid Preparation

Journal: Human reproduction (Oxford, England)

Article Title: Activin A regulates trophoblast cell adhesive properties: implications for implantation failure in women with endometriosis-associated infertility.

doi: 10.1093/humrep/deq097

Figure Lengend Snippet: Figure 2 pSmad2 of HTR8 trophoblast cells.(A) Treatment of HTR8 trophoblast cells with activin A (ActA; 1, 10, 50 and 100 ng/ml) for 24 h results in a dose-dependent increase in pSmad2 as shown by western blot. Addition of activin inhibitor, SB431542 (SB: 10 mM) in conjunction with ActA (50 ng/ml), decreased pSmad2 compared with ActA alone. The lower band represents total Smad2 which is not changed with ActA treatment. (B) Densitometric analysis of representative western blot in (A). Each bar represents the mean of two experiments assayed in duplicate.

Article Snippet: Membranes were exposed to autoradiography film (Hyperfilm ECL; GE Healthcare) for 5 min. Membranes were then washed in stripping buffer (Re Blot Plus, by guest on N ovem ber 16, 2015 http://hum rep.oxfordjournals.org/ D ow nloaded from Chemicon International, CA, USA) before blocking again in 10% skim milk/TBS, overnight incubation with rabbit anti-human Smad2 (86F7; Cell Signalling Technology, #3122; 1:1000 in 5% skim milk/TBS) and secondary antibody and detection as described above.

Techniques: Western Blot